SNRPB2 Monoklonaler Antikörper

SNRPB2 Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

1D11E9

Kat-Nr. : 68095-1-PBS

Synonyme

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

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  WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68095-1-PBS bindet in Indirect ELISA, WB SNRPB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SNRPB2 fusion protein Ag5540
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide B''
• Berechnetes Molekulargewicht: 225 aa, 26, 5 kDa
• Beobachtetes Molekulargewicht: 25-31 kDa
• GenBank-Zugangsnummer: BC036737
• Gene symbol: SNRPB2
• Gene ID (NCBI): 6629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.