SNRPE Polyklonaler Antikörper

SNRPE Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20407-1-AP

Synonyme

snRNP-E, snRNP E, Sm-E, SME, Sm protein E

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  WB analysis using 20407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20407-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20407-1-AP (SNRPE antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 20407-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Plazenta-Gewebe, humanes Hirngewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20407-1-AP bindet in WB, IHC, IF, ELISA SNRPE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNRPE fusion protein Ag14227
• Vollständiger Name: small nuclear ribonucleoprotein polypeptide E
• Berechnetes Molekulargewicht: 92 aa, 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC002639
• Gene symbol: SNRPE
• Gene ID (NCBI): 6635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The snRNP E (SNRPE)protein is one of several proteins associated with the U family of small nuclear RNAs that are involved in RNA processing. It functions in the U7 snRNP complex that is involved in histone 3'-end processing and also associated with snRNP U1, U2, U4/U6 and U5. This antibody raises against the full length of human SNRPE, and can recognize two bands of SNRPE, include a 9 kDa band(isoform) and a 11kDa band(SNRPE) (PMID:23246290)

Publikationen

Species	Application	Title
human	IF	Am J Hum Genet; Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.; Authors - Sandra M Pasternack
human	WB,IHC	J Pathol; A novel protein-based prognostic signature improves risk stratification to guide clinical management in early-stage lung adenocarcinoma patients.; Authors - Elena Martínez-Terroba
human	WB	Front Immunol; Multi-omics analysis and experiments uncover the function of cancer stemness in ovarian cancer and establish a machine learning-based model for predicting immunotherapy responses; Authors - Zhibing Liu ; KD Validated