SNX1 Rekombinanter Antikörper

SNX1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251062D3

Kat-Nr. : 86335-3-RR

Synonyme

Sorting nexin-1

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  WB analysis using 86335-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86335-3-RR (SNX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 86335-3-RR

  IP result of anti-SNX1 (IP:86335-3-RR, 4ug; Detection:86335-3-RR 1:2000) with HeLa cells lysate 1400 ug.

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  Affinity and Kinetic Characterization of 86335-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86335-3-RR against Human SNX1 were performed. The affinity constant is 0.921 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Ramos-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86335-3-RR bindet in WB, ELISA SNX1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SNX1 fusion protein Ag0499
• Vollständiger Name: sorting nexin 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC000357
• Gene symbol: SNX1
• Gene ID (NCBI): 6642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexin 1 (SNX1, synonyms: SNX1A, MGC8664, HsT17379) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. SNX1 also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members.