SNX15 Polyklonaler Antikörper

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• KD/KO Validated

SNX15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16049-1-AP

Synonyme

HSAF001435, sorting nexin 15, Sorting nexin-15

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  WB analysis using 16049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16049-1-AP

  WB result of SNX15 antibody (16049-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX15 transfected HeLa cells.

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  WB analysis using 16049-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16049-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16049-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 16049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, humanes Hodengewebe, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16049-1-AP bindet in WB, IHC, ELISA SNX15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX15 fusion protein Ag9006
• Vollständiger Name: sorting nexin 15
• Berechnetes Molekulargewicht: 342 aa, 38 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC009897
• Gene symbol: SNX15
• Gene ID (NCBI): 29907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.