SNX17 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNX17 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 10275-1-AP

Synonyme

KIAA0064, Sorting nexin-17

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  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10275-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10275-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10275-1-AP

  IP result of anti-SNX17 (IP:10275-1-AP, 4ug; Detection:10275-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Maushautgewebe, RAW 264.7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 21 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

10275-1-AP bindet in WB, IHC, IF, IP, ELISA SNX17 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX17 fusion protein Ag0355
• Vollständiger Name: sorting nexin 17
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002524
• Gene symbol: SNX17
• Gene ID (NCBI): 9784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin.

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
mouse	WB	Nat Cell Biol; Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.; Authors - B?ttcher Ralph Thomas RT ; KD Validated
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Proc Natl Acad Sci U S A; LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.; Authors - Björn Schorch ; KD Validated

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