SNX17 Polyklonaler Antikörper

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• KD/KO Validated

SNX17 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 10275-1-AP

Synonyme

KIAA0064, SNX17, sorting nexin 17

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Galerie der Validierungsdaten

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  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 10275-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10275-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10275-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10275-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10275-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10275-1-AP

  IP Result of anti-SNX17 (IP:10275-1-AP, 4ug; Detection:10275-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Maushautgewebe, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 18 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

10275-1-AP bindet in WB, IP, IHC, IF, ELISA SNX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX17 fusion protein Ag0355
• Vollständiger Name: sorting nexin 17
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002524
• Gene symbol: SNX17
• Gene ID (NCBI): 9784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
mouse	WB	Nat Cell Biol; Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.; Authors - B?ttcher Ralph Thomas RT ; KD Validated
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Proc Natl Acad Sci U S A; LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.; Authors - Björn Schorch ; KD Validated

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