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SNX18 Rekombinanter Antikörper

SNX18 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241537D11

Kat-Nr. : 84215-4-PBS

Synonyme

Sorting nexin-18, SH3PXD3B, SH3 and PX domain-containing protein 3B, 241537D11

Filter:
  • Western Blot (WB) analysis of various lysates using SNX18 Recombinant antibody (84215-4-RR)

    WB analysis using 84215-4-RR (same clone as 84215-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SNX18 Recombinant antibody (84215-4-RR)

    WB analysis of mouse brain using 84215-4-RR (same clone as 84215-4-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 84215-4-RR (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNX18 Recombinant antibody (84215-4-RR)

    IF Staining of MCF-7 using 84215-4-RR (same clone as 84215-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SNX18 antibody (84215-4-RR, Clone: 241537D11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84215-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84215-4-RR

    Affinity and Kinetic Characterization of 84215-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84215-4-RR against Human SNX18 were performed. The affinity constant is 0.378 nM.

Geprüfte Anwendungen

Produktinformation

84215-4-PBS bindet in WB, IF/ICC, Indirect ELISA SNX18 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SNX18 fusion protein Ag16645
Vollständiger Name sorting nexin 18
Berechnetes Molekulargewicht 628 aa, 69 kDa
Beobachtetes Molekulargewicht68-70 kDa
GenBank-ZugangsnummerBC060791
Gene symbol SNX18
Gene ID (NCBI) 112574
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).