SNX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNX3 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 10772-1-AP

Synonyme

Grd19, MCOPS8, Protein SDP3, SDP3, SNX3, sorting nexin 3

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Galerie der Validierungsdaten

• 

  WB analysis of COLO 320 using 10772-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10772-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10772-1-AP

  Immunofluorescent analysis of HeLa cells using 10772-1-AP (SNX3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

10772-1-AP bindet in WB, IHC, IF, CoIP, ELISA SNX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX3 fusion protein Ag1160
• Vollständiger Name: sorting nexin 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC008444
• Gene symbol: SNX3
• Gene ID (NCBI): 8724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.; Authors - Fergal O'Farrell ; KD Validated
mouse	WB,IHC,IF,CoIP	Acta Pharm Sin B; Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis; Authors - Wenjing Yu
human	WB,IF	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough ; KD Validated
mouse,rat	IF,WB	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu ; KO Validated
human	WB,IF	Oncogene; EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.; Authors - Esra Cicek ; KD Validated
mouse	WB	J Proteomics; Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.; Authors - Fang Jiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rafeeq (Verified Customer) (01-30-2019)

I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs

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