SNX3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNX3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 10772-1-AP

Synonyme

Grd19, MCOPS8, Protein SDP3, SDP3, sorting nexin 3

---

• 

  WB analysis of COLO 320 using 10772-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10772-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10772-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10772-1-AP (SNX3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10772-1-AP (SNX3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10772-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10772-1-AP (SNX3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10772-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX3 antibody (10772-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A431 using 10772-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (10772-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 2 publications below

Produktinformation

10772-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SNX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX3 fusion protein Ag1160
• Vollständiger Name: sorting nexin 3
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC008444
• Gene symbol: SNX3
• Gene ID (NCBI): 8724
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.; Authors - Fergal O'Farrell ; KD Validated
mouse	WB,IHC,IF,CoIP	Acta Pharm Sin B; Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis; Authors - Wenjing Yu
human	WB,IF	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough ; KD Validated
mouse	WB	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
rat,mouse	WB,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu ; KO Validated
human	WB,IF	Oncogene; EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.; Authors - Esra Cicek ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Rafeeq (Verified Customer) (01-30-2019)

I have been using Antibodies from Proteintech since two years. They have antibodies reactive to both mouse and human origin proteins. This is always a plus point.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MEFs