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  • Featured Product
  • KD/KO Validated

SNX3 Monoklonaler Antikörper

SNX3 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B5B7

Kat-Nr. : 68292-1-Ig

Synonyme

2B5B7, Grd19, MCOPS8, Protein SDP3, SDP3

Filter:
  • Western Blot (WB) analysis of various lysates using SNX3 Monoclonal antibody (68292-1-Ig)

    WB analysis using 68292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68292-1-Ig (SNX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNX3 Monoclonal antibody (68292-1-Ig)

    IF Staining of A431 using 68292-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX3 antibody (68292-1-Ig, Clone: 2B5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCA431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68292-1-Ig bindet in WB, IF/ICC, ELISA SNX3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SNX3 fusion protein Ag1160
Vollständiger Name sorting nexin 3
Berechnetes Molekulargewicht 19 kDa
Beobachtetes Molekulargewicht16-20 kDa
GenBank-ZugangsnummerBC008444
Gene symbol SNX3
Gene ID (NCBI) 8724
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SNX3 antibody 68292-1-IgProtokoll herunterladen
IF protocol for SNX3 antibody 68292-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ioana (Verified Customer) (08-28-2025)

This antibody gave a good strong band in Western Blotting, allowing me to calculate the amount of overexpression and siRNA knock-down efficiency in my cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLaM