SNX32 Polyklonaler Antikörper

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• KD/KO Validated

SNX32 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25763-1-AP

Synonyme

SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B

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  WB analysis of HeLa using 25763-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25763-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of mouse brain using 25763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25763-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA SNX32 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX32 fusion protein Ag22653
• Vollständiger Name: sorting nexin 32
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC040981
• Gene symbol: SNX32
• Gene ID (NCBI): 254122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX32 (Sorting nexin-32, also known as SNX6B) may be involved in several stages of intracellular trafficking.

Publikationen

Species	Application	Title
human,pig	CoIP,WB,IF,IP	J Virol; SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway; Authors - Wenping Yang ; KD Validated