SNX5 Polyklonaler Antikörper

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• KD/KO Validated

SNX5 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 17918-1-AP

Synonyme

Sorting nexin-5

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  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17918-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 17918-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17918-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17918-1-AP (SNX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 17918-1-AP

  IP result of anti-SNX5 (IP:17918-1-AP, 4ug; Detection:17918-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17918-1-AP (SNX5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 17918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17918-1-AP (SNX5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, humanes Nierengewebe, Jurkat-Zellen, K-562-Zellen, Mausnierengewebe, Neuro-2a-Zellen, U2OS-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

17918-1-AP bindet in WB, IHC, IP, ELISA SNX5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX5 fusion protein Ag12330
• Vollständiger Name: sorting nexin 5
• Berechnetes Molekulargewicht: 404 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC093623
• Gene symbol: SNX5
• Gene ID (NCBI): 27131
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	WB	Nat Commun; SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.; Authors - Ian J McGough
mouse	WB	Proc Natl Acad Sci U S A; Retromer dysfunction in amyotrophic lateral sclerosis.; Authors - Eduardo J Pérez-Torres
human	WB	J Cell Biol; Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.; Authors - Boris Simonetti ; KO Validated
rat	WB	Oxid Med Cell Longev; Sorting Nexin 5 Plays an Important Role in Promoting Ferroptosis in Parkinson's Disease.; Authors - Zifeng Huang ; KD Validated
human	WB	Cell Rep; The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking; Authors - Khavong Pha