SNX6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SNX6 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10114-1-AP

Synonyme

MSTP010, SNX6, sorting nexin 6, TFAF2, TRAF4 associated factor 2

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Galerie der Validierungsdaten

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  WB analysis using 10114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10114-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10114-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10114-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10114-1-AP (SNX6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10114-1-AP

  IP Result of anti-SNX6 (IP:10114-1-AP, 4ug; Detection:10114-1-AP 1:600) with HepG2 cells lysate 4000ug.

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  IHC staining of mouse brain using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10114-1-AP (SNX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 10114-1-AP (SNX6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10114-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNX6 antibody (10114-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of RAW 264.7 using 10114-1-AP

  1X10^6 RAW 264.7 cells were stained with 0.2ug SNX6 antibody (10114-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Maushirngewebe, RAW 264.7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

10114-1-AP bindet in WB, IP, IHC, IF, FC, ELISA SNX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX6 fusion protein Ag0168
• Vollständiger Name: sorting nexin 6
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC001798
• Gene symbol: SNX6
• Gene ID (NCBI): 58533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); SNX6 predicts poor prognosis and contributes to the metastasis of pancreatic cancer cells via activating epithelial-mesenchymal transition.; Authors - Pengfei Hu ; KD Validated
human	WB	Front Immunol; Zika virus modulates mitochondrial dynamics, mitophagy, and mitochondria-derived vesicles to facilitate viral replication in trophoblast cells; Authors - Jae Kyung Lee