SNX9 Polyklonaler Antikörper

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• KD/KO Validated

SNX9 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 15721-1-AP

Synonyme

SH3PX1, Protein SDP1, SDP1, SH3 and PX domain-containing protein 1, SH3 and PX domain-containing protein 3A

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  WB analysis using 15721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15721-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15721-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15721-1-AP (SNX9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15721-1-AP

  IP result of anti-SNX9 (IP:15721-1-AP, 4ug; Detection:15721-1-AP 1:8000) with mouse heart tissue lysate 1880 ug.

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  IHC staining of human stomach cancer using 15721-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15721-1-AP (SNX9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15721-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX9 antibody (15721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15721-1-AP

  Immunofluorescent analysis of HeLa cells using 15721-1-AP (SNX9 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

15721-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SNX9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX9 fusion protein Ag8440
• Vollständiger Name: sorting nexin 9
• Berechnetes Molekulargewicht: 595 aa, 67 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC005022
• Gene symbol: SNX9
• Gene ID (NCBI): 51429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

Publikationen

Species	Application	Title
human	WB	Immunity; cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity; Authors - Yunlong Zhao
monkey,human	WB,IF	Mol Cell; β-hydroxybutyrate facilitates mitochondrial-derived vesicle biogenesis and improves mitochondrial functions; Authors - Min Tang ; KO Validated
mouse	WB	Nat Commun; Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.; Authors - Kiran Todkar ; KD Validated
human	IF	Cancer Discov; In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes.; Authors - Chen Li Chew
human	WB	Dev Cell; Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.; Authors - Christina G Towers ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki