SOCS6 Polyklonaler Antikörper

SOCS6 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27343-1-AP

Synonyme

CIS 4, CIS4, CIS-4, Cytokine-inducible SH2 protein 4, HSPC060

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  WB analysis using 27343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27343-1-AP (SOCS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 27343-1-AP

  IP result of anti-SOCS6 (IP:27343-1-AP, 4ug; Detection:27343-1-AP 1:1500) with NIH/3T3 cells lysate 1200 ug.

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  IP experiment of human placenta using 27343-1-AP

  IP result of anti-SOCS6 (IP:27343-1-AP, 4ug; Detection:27343-1-AP 1:1500) with human placenta tissue lysate 960 ug.

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  IHC staining of human colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 27343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27343-1-AP (SOCS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Plazenta-Gewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27343-1-AP bindet in WB, IHC, IP, ELISA SOCS6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOCS6 fusion protein Ag25594
• Vollständiger Name: suppressor of cytokine signaling 6
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-75 kDa
• GenBank-Zugangsnummer: BC020082
• Gene symbol: SOCS6
• Gene ID (NCBI): 9306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOCS6 (Suppressor of cytokine signaling 6) is also named as CIS4 and SOCS4. SOCS6 is one of eight members of the SOCS family of proteins. SOCS6 is involved in negative regulation of receptor signaling by increasing degradation mediated by ubiquitination of receptors or substrate proteins and induces apoptosis by targeting mitochondrial proteins (PMID: 25172101). SOCS6 is widely expressed in many tissues and is found to be downregulated in many cancers including colorectal cancer, gastric cancer, lung cancer, ovarian cancer, stomach cancer, thyroid cancer, hepatocellular carcinoma, and pancreatic cancer (PMID: 25172101). In addition to its molecular weight of 60 kDa, SOCS6 has a 70 kDa isoform (PMID: 34169835, PMID: 22125600).