SOD1 Rekombinanter Antikörper

SOD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240888B9

Kat-Nr. : 83857-6-RR

Synonyme

IPOA, hSod1, homodimer, EC:1.15.1.1, ALS1

---

• 

  WB analysis using 83857-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83857-6-RR (SOD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83857-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83857-6-RR against Human SOD1 were performed. The affinity constant is 13.0 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83857-6-RR bindet in WB, ELISA SOD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: superoxide dismutase 1, soluble
• Berechnetes Molekulargewicht: 16kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: NM_000454.5
• Gene symbol: SOD1
• Gene ID (NCBI): 6647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.