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SOD2 Monoklonaler Antikörper
SOD2 Monoklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
IF/ICC, FC (Intra)
Konjugation
CoraLite®555 Fluorescent Dye
CloneNo.
3A6C2
Kat-Nr. : CL555-66474
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | A549-Zellen |
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL555-66474 bindet in IF/ICC, FC (Intra) SOD2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | SOD2 fusion protein Ag21388 |
| Vollständiger Name | superoxide dismutase 2, mitochondrial |
| Berechnetes Molekulargewicht | 25 kDa |
| Beobachtetes Molekulargewicht | 25 kDa |
| GenBank-Zugangsnummer | BC016934 |
| Gene symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| Konjugation | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570 nm |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL555 SOD2 antibody CL555-66474 | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Anja (Verified Customer) (07-25-2022) | The antibody worked well for Flow Cytometry at a 1:100 dilution for isolated mouse cells. I would buy it again.
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