SORBS1 Polyklonaler Antikörper

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• KD/KO Validated

SORBS1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13854-1-PBS

Synonyme

CAP, c-Cbl-associated protein, SH3D5, SORB1, Sorbin and SH3 domain-containing protein 1

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  WB analysis of 3T3-L1 using 13854-1-AP (same clone as 13854-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13854-1-AP (same clone as 13854-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 13854-1-AP (same clone as 13854-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 13854-1-AP (same clone as 13854-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 13854-1-AP (same clone as 13854-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 13854-1-AP (same clone as 13854-1-PBS)

  IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug. This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 13854-1-AP (same clone as 13854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 13854-1-AP (same clone as 13854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13854-1-AP (SORBS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13854-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13854-1-PBS bindet in WB, IHC, IP, Indirect ELISA SORBS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SORBS1 fusion protein Ag4828
• Vollständiger Name: sorbin and SH3 domain containing 1
• Berechnetes Molekulargewicht: 1292 aa, 142 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC042612
• Gene symbol: SORBS1
• Gene ID (NCBI): 10580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The sorbin homology (SoHo) family of adapter and scaffold proteins consists of three proteins: CAP, also known as Sorbin and SH3 domain containing 1 (Sorbs1), ArgBP2, also known as Sorbs2, and Vinexin, also known as Sorbs3. All Sorbs proteins are highly expressed in heart tissue, skeletal muscle, adipose tissue, and cells of the immune system, functioning as SH3-domain-mediated adaptors of scaffolding molecules(PMID: 32738595). SORBS1 can bind to signaling molecules (e.g., c-Cbl, c-Abl, and insulin reporter) to regulate glucose transport, transcription activity, and insulin signaling(PMID: 27791200).