SORBS2 Polyklonaler Antikörper

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• KD/KO Validated

SORBS2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 24643-1-AP

Synonyme

Arg/Abl-interacting protein 2, Arg-binding protein 2, ArgBP2, Sorbin and SH3 domain-containing protein 2

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  WB analysis using 24643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 24643-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 24643-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 24643-1-AP

  IP result of anti-SORBS2 (IP:24643-1-AP, 4ug; Detection:24643-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 24643-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, MDCK-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe, U-251-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below

Produktinformation

24643-1-AP bindet in WB, IHC, IF, IP, ELISA SORBS2 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SORBS2 fusion protein Ag20041
• Vollständiger Name: sorbin and SH3 domain containing 2
• Berechnetes Molekulargewicht: 1100 aa, 124 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC011883
• Gene symbol: SORBS2
• Gene ID (NCBI): 8470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).

Publikationen

Species	Application	Title
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB,IP,IHC,IF	EBioMedicine; Elevated myocardial SORBS2 and the underlying implications in left ventricular noncompaction cardiomyopathy.; Authors - Chunyan Li
mouse,human	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB,IP	J Biol Chem; Arg Kinase Binding Protein 2 (ArgBP2) interaction with α-actinin and actin stress fibres inhibits cell migration.; Authors - Praju Vikas Anekal
human	WB	J Transl Med; SORBS2 as a molecular target for atherosclerosis in patients with familial hypercholesterolemia.; Authors - Ming-Ming Liu
human	WB	Mol Med Rep; Musk ketone induces apoptosis of gastric cancer cells via downregulation of sorbin and SH3 domain containing 2.; Authors - Juan An ; KD Validated