SORBS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SORBS2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24643-1-PBS

Synonyme

---

• 

  WB analysis using 24643-1-AP (same clone as 24643-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 24643-1-AP (same clone as 24643-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDCK using 24643-1-AP (same clone as 24643-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 24643-1-AP (SORBS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 24643-1-AP (same clone as 24643-1-PBS)

  IP result of anti-SORBS2 (IP:24643-1-AP, 4ug; Detection:24643-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 24643-1-AP (same clone as 24643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 24643-1-AP (same clone as 24643-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 24643-1-AP (SORBS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24643-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

24643-1-PBS bindet in WB, IHC, IP, Indirect ELISA SORBS2 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SORBS2 fusion protein Ag20041
• Vollständiger Name: sorbin and SH3 domain containing 2
• Berechnetes Molekulargewicht: 1100 aa, 124 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC011883
• Gene symbol: SORBS2
• Gene ID (NCBI): 8470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORBS2, also known as ARGBP2, has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. SORBS2 is widely expressed in human tissues and extremely abundant in the heart. In epithelial cells SORBS2 is located in stress fibers and the nucleus; In cardiac muscle cells. SORBS2 is located in the Z-disks of sarcomeres. The subcellular localization suggests that SORBS2 functions as an adapter protein to assemble signaling complexes in stress fibers, and may influence the contractile or elastic properties of cardiac sarcomeres(PMID: 9211900). Alternate splicing results in multiple transcript variants( PMID: 28961272).