SORCS2 Polyklonaler Antikörper

SORCS2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 55063-1-AP

Synonyme

KIAA1329, SorCS2 104 kDa chain, SorCS2 122 kDa chain, SorCS2 18 kDa chain, VPS10 domain-containing receptor SorCS2

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  WB analysis of Jurkat using 55063-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55063-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55063-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HaCaT using 55063-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using SORCS2 antibody (55063-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

55063-1-AP bindet in WB, IF/ICC, ELISA SORCS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sortilin-related VPS10 domain containing receptor 2
• Berechnetes Molekulargewicht: 128 kDa
• Beobachtetes Molekulargewicht: 128-140 kDa
• GenBank-Zugangsnummer: NM_020777
• Gene symbol: SORCS2
• Gene ID (NCBI): 57537
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SORCS2, also named as KIAA1329, is one of the VPS10 domain-containing receptors. It contains 6 BNR repeats and 1 PKD domain.. The antibody is specific to SORCS2.

Publikationen

Species	Application	Title
rat	WB	Cytojournal; Astragalus polysaccharide attenuates retinal ischemia reperfusion-induced microglial activation through sortilin-related vacuolar protein sorting 10 domain containing receptor 2/laminin subunit alpha 1 upregulation; Authors - Juanjuan Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juan Pablo (Verified Customer) (11-29-2018)

I tried with different lysis buffer. I tried blocking with 5% milk or 2% BSA. Never worked for me. Didn't work for immunohistochemistry either dilution 1:1000 and 1:100.

• Applications: Western Blot, Immunohistochemistry,
• Primary Antibody Dilution: 1-500
• Cell Tissue Type: Postnatal cerebellum, from P2 to P14

SORCS2 Antibody Western Blot,Immunohistochemistry, validation (1-500 dilution) in Postnatal cerebellum, from P2 to P14 (Cat no:55063-1-AP)