SORLA Monoklonaler Antikörper

SORLA Monoklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E7D9

Kat-Nr. : 68651-1-Ig

Synonyme

SORL1, SORL 1, Low-density lipoprotein receptor relative with 11 ligand-binding repeats, LDLR relative with 11 ligand-binding repeats, C11orf32

---

• 

  WB analysis using 68651-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68651-1-Ig (SORL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse cerebellum using 68651-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SORL1 antibody (68651-1-Ig, Clone: 2E7D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen
• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68651-1-Ig bindet in WB, IF-P, ELISA SORLA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SORLA fusion protein Ag18445
• Vollständiger Name: sortilin-related receptor, L(DLR class) A repeats-containing
• Berechnetes Molekulargewicht: 2214 aa, 248 kDa
• Beobachtetes Molekulargewicht: 300 kDa
• GenBank-Zugangsnummer: BC137171
• Gene symbol: SORLA
• Gene ID (NCBI): 6653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SorLA also known as SorL1 or LR11, is a multiligand receptor belonging to the LDL receptor superfamily. It is also a member of vacuolar protein sorting-10 (Vps10) family and is enriched in the brain. SorLA acts as an sorting receptor for APP and prevents amyloidogenic processing of APP. Downregulation of SorLA has been found in patients with sporadic Alzheimer disease (AD). Currently SorLA has emerged as a potential target for AD therapy. (25404298)