SOST/Sclerostin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SOST/Sclerostin Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 21933-1-AP

Synonyme

SOST, sclerosteosis, Sclerostin, SOST1, UNQ2976/PRO7455/PRO7476

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  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (SOST/Sclerostin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (SOST/Sclerostin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (SOST/Sclerostin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (SOST/Sclerostin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21933-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Sclerostin antibody (21933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe, Mausembryogewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Produktinformation

21933-1-AP bindet in WB, IHC, IF/ICC, ELISA SOST/Sclerostin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOST/Sclerostin fusion protein Ag16567
• Vollständiger Name: sclerosteosis
• Berechnetes Molekulargewicht: 213 aa, 24 kDa
• GenBank-Zugangsnummer: BC101086
• Gene symbol: Sclerostin
• Gene ID (NCBI): 50964
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease

Publikationen

Species	Application	Title
mouse	IF	EMBO Mol Med; Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2.; Authors - Xiaoan Wei
rat	WB	Aging (Albany NY); Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure.; Authors - Fang He
human	WB,IHC	Front Oncol; Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.; Authors - Hanqing Wang ; KD Validated
mouse	IHC	Ann Hematol; Higher Sclerostin/SOST expression is associated with lower percentage of circulatory blasts and better prognosis in patients with myelofibrosis.; Authors - Marko Lucijanic
rat	IHC	Clin Oral Investig; Mechanisms of sphingosine-1-phosphate (S1P) signaling on excessive stress-induced root resorption during orthodontic molar intrusion.; Authors - Han Wang
mouse	WB	J Orthop Surg Res; Lysyl oxidase inhibits BMP9-induced osteoblastic differentiation through reducing Wnt/β-catenin via HIF-1a repression in 3T3-L1 cells; Authors - Jie Zhang