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SOX10 Monoklonaler Antikörper

SOX10 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C8

Kat-Nr. : CL488-66786

Synonyme

DOM, SOX10, Transcription factor SOX 10, WS2E, WS4

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    IF Staining of mouse brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    IF Staining of rat brain using CL488-66786

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite® Plus 488 SOX10 antibody (CL488-66786, Clone: 1D2C8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of C6 cells using CoraLite® Plus 488-conjugated SOX10 Monoclonal ant (CL488-66786)

    FC experiment of C6 using CL488-66786

    1X10^6 C6 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SOX10 (CL488-66786, Clone:1D2C8) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in FCC6-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66786 bindet in IF, FC (Intra) SOX10 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SOX10 fusion protein Ag23191
Vollständiger Name SRY (sex determining region Y)-box 10
Berechnetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC002824
Gene symbol SOX10
Gene ID (NCBI) 6663
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 SOX10 antibody CL488-66786Protokoll herunterladen
FC protocol for CL Plus 488 SOX10 antibody CL488-66786Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen