SOX13 Polyklonaler Antikörper

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• KD/KO Validated

SOX13 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18902-1-AP

Synonyme

ICA12, Islet cell antigen 12, Sox 13, SOX13, Transcription factor SOX 13

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Galerie der Validierungsdaten

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  WB analysis using 18902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 18902-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 18902-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18902-1-AP (SOX13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18902-1-AP

  IP Result of anti-SOX13 (IP:18902-1-AP, 4ug; Detection:18902-1-AP 1:500) with SH-SY5Y cells lysate 1200ug.

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  IF Staining of HeLa using 18902-1-AP

  Immunofluorescent analysis of Hela cells, using SOX13 antibody 18902-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BGC-823-Zellen, NCI-H1299-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

18902-1-AP bindet in WB, IP, IHC, IF, chIP, ELISA SOX13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX13 fusion protein Ag13537
• Vollständiger Name: SRY (sex determining region Y)-box 13
• Berechnetes Molekulargewicht: 622 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC106038
• Gene symbol: SOX13
• Gene ID (NCBI): 9580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRY-BOX 13(SOX13), identified a sequence homologous more than 60% to the SRY HMG box ,with a centrally located HMG box and an N-terminal leucine zipper motif with a neighboring glutamine stretch called a Q box. SOX13 binds to the consensus HMG-box motif. It's an IDDM-specific human autoantigen, and a gamma-delta-specific gene in the immune system. It can modulates Wnt/TCF activity. This is a rabbit polyclonal raised against part of N-terminal chain of SOX13 of human origin.

Publikationen

Species	Application	Title
human	WB,IF	Front Cardiovasc Med; Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells; Authors - Catherine Demos ; KD Validated
human	WB,IHC	Lab Invest; Sox13 promotes hepatocellular carcinoma metastasis by transcriptionally activating Twist1.; Authors - Min Feng ; KD Validated
mouse	WB, IF	Mol Cell Biochem; Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development.; Authors - Mikella Daigle
human	WB,IHC	Bioengineered; Sex-determining Region Y-box transcription factor 13 promotes breast cancer cell proliferation and glycolysis by activating the tripartite motif containing 11-mediated Wnt/β-catenin signaling pathway.; Authors - Xiaoyan Jin ; KD Validated
human	WB,chIP	J Exp Clin Cancer Res; FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.; Authors - Zhenwei He