SOX17 Polyklonaler Antikörper

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• KD/KO Validated

SOX17 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 24903-1-AP

Synonyme

SOX17, Transcription factor SOX 17

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Galerie der Validierungsdaten

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  WB analysis using 24903-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 24903-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24903-1-AP (SOX17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 7 publications below

Produktinformation

24903-1-AP bindet in WB, IF, ELISA SOX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX17 fusion protein Ag19157
• Vollständiger Name: SRY (sex determining region Y)-box 17
• Berechnetes Molekulargewicht: 414 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC111365
• Gene symbol: SOX17
• Gene ID (NCBI): 64321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcription factor SOX 17 (SOX17) also named as SRY box 17 is a 414 amino acid protein, which contains one Sox C-terminal domain and one HMG box DNA-binding domain. SOX17 as a transcription regulator binds target promoter DNA and bend the DNA via WNT3A. SOX17 is a marker of endodermal cells and a transcriptional regulator containing a DNA binding domain called the HMG box. In mouse embryos, SOX17 plays critical roles in the growth and differentiation of definitive endodermal cells (PMID: 11973269), the remodeling of endothelial cells (PMID: 16895970), hindgut endoderm expansion with localization of primordial germ cells (PMID: 19371732), and gallbladder/bile duct formation (PMID: 19913509).

Publikationen

Species	Application	Title
mouse	WB	Stem Cell Reports; Phosphorylation of Threonine343 Is Crucial for OCT4 Interaction with SOX2 in the Maintenance of Mouse Embryonic Stem Cell Pluripotency.; Authors - Xianmixinuer Abulaiti
mouse	WB	Front Cell Dev Biol; ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.; Authors - XiaoYun Sun
human	IF	Stem Cell Res; Establishment of an arrhythmogenic right ventricular cardiomyopathy derived iPSC cell line (USFi004-A) carrying a heterozygous mutation in PKP2 (c.1799delA).; Authors - Jiajia Yang
human	IF	Stem Cell Res; Generation of a Friedreich's Ataxia patient-derived iPSC line USFi001-A.; Authors - Mariana Burgos Angulo
human	IF	Stem Cell Res; Generation of an iPSC cell line (USFi003-A) from a patient with dilated cardiomyopathy carrying a heterozygous mutation in LMNA (p.R541C).; Authors - Jiajia Yang
human	IF	Stem Cell Res; Generation of a heterozygous FLNC mutation-carrying human iPSC line, USFi002-A, for modeling dilated cardiomyopathy.; Authors - Mariana A Argenziano