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SOX2 Rekombinanter Antikörper

SOX2 Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230225G1

Kat-Nr. : 83059-5-PBS

Synonyme

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

Filter:
  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Recombinant antibody (83059-5-RR)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Recombinant antibody (83059-5-RR)

    IF Staining of U-251 using 83059-5-RR (same clone as 83059-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (83059-5-RR, Clone: 230225G1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83059-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83059-5-RR

    Affinity and Kinetic Characterization of 83059-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83059-5-RR against Human SOX2 were performed. The affinity constant is 3.54 nM.

Geprüfte Anwendungen

Produktinformation

83059-5-PBS bindet in IF/ICC, Indirect ELISA SOX2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen SOX2 fusion protein Ag0612
Vollständiger Name SRY (sex determining region Y)-box 2
Berechnetes Molekulargewicht 34 kDa
GenBank-ZugangsnummerBC013923
Gene symbol SOX2
Gene ID (NCBI) 6657
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.