SOX7 Polyklonaler Antikörper

SOX7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 23925-1-AP

Synonyme

Transcription factor SOX-7, Transcription factor SOX 7, SOX 7, endoderm lineage markers

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  WB analysis using 23925-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 23925-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 23925-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 23925-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23925-1-AP (SOX7 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 23925-1-AP (SOX7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 23925-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23925-1-AP (SOX7 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U2OS using 23925-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SOX7 antibody (23925-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Mausherzgewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

23925-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SOX7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOX7 fusion protein Ag10323
• Vollständiger Name: SRY (sex determining region Y)-box 7
• Berechnetes Molekulargewicht: 388 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC071947
• Gene symbol: SOX7
• Gene ID (NCBI): 83595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification.

Publikationen

Species	Application	Title
human	WB	Oncogene; SOX7 regulates MAPK/ERK-BIM mediated apoptosis in cancer cells.; Authors - Qiao-Yang Sun
human,mouse	WB,IF	FASEB J; System analysis based on weighted gene co-expression analysis identifies SOX7 as a novel regulator of hepatic stellate cell activation and liver fibrosis; Authors - Yuwei Liu
human	WB,IP	Mol Genet Genomics; Predisposition to atrioventricular septal defects may be caused by SOX7 variants that impair interaction with GATA4.; Authors - Baolei Li
human	WB	Cell Death Dis; The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling.; Authors - Nanchao Hong
human	WB,IF	Int J Biol Macromol; Integrated transcriptomics and lipidomics reveals protective effect in vascular endothelial barrier of a polysaccharide from Typhae Pollen; Authors - Mingliang Gao
mouse	WB	J Mol Cell Cardiol; Deficiency of Sox7 leads to congenital aortic stenosis via abnormal valve remodeling; Authors - Weiqi Feng