SOX8 Rekombinanter Antikörper

SOX8 Rekombinant Antikörper für IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240120H6

Kat-Nr. : 83238-3-PBS

Synonyme

240120H6, Transcription factor SOX 8, Transcription factor SOX-8

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  IF Staining of mouse brain using 83238-3-RR (same clone as 83238-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83238-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83238-3-RR (same clone as 83238-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83238-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00222-1

  Cytometric bead array standard curve of MP00222-1, SOX8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83238-1-PBS. Detection antibody: 83238-3-PBS. Standard: Ag34395. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00222-2

  Cytometric bead array standard curve of MP00222-2, SOX8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83238-3-PBS. Detection antibody: 83238-2-PBS. Standard: Ag34395. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83238-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.

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Geprüfte Anwendungen

Produktinformation

83238-3-PBS bindet in IF/ICC, IF-P, Cytometric bead array, Indirect ELISA SOX8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SOX8 fusion protein Ag34395
• Vollständiger Name: SRY (sex determining region Y)-box 8
• Berechnetes Molekulargewicht: 446 aa, 47 kDa
• GenBank-Zugangsnummer: BC031797
• Gene symbol: SOX8
• Gene ID (NCBI): 30812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.