SP1 Monoklonaler Antikörper

SP1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

4G3H3

Kat-Nr. : 66508-1-PBS

Synonyme

4G3H3, SP 1, Sp1 transcription factor, Specificity protein 1, TSFP1

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  WB analysis using 66508-1-Ig (same clone as 66508-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66508-1-Ig (same clone as 66508-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66508-1-Ig (same clone as 66508-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SP1 (66508-1-Ig, Clone:4G3H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66508-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66508-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA SP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SP1 fusion protein Ag16720
• Vollständiger Name: Sp1 transcription factor
• Berechnetes Molekulargewicht: 785 aa, 81 kDa
• Beobachtetes Molekulargewicht: 95-105 kDa, 60-65 kDa
• GenBank-Zugangsnummer: BC062539
• Gene symbol: SP1
• Gene ID (NCBI): 6667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].