SP100 Rekombinanter Antikörper

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• KD/KO Validated

SP100 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241153F8

Kat-Nr. : 84012-5-RR

Synonyme

241153F8, Nuclear autoantigen Sp-100, Nuclear dot-associated Sp100 protein, SP 100, SP100 nuclear antigen

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  WB analysis using 84012-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84012-5-RR (SP100 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 84012-5-RR

  WB result of SP100 antibody (84012-5-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-SP100 transfected A431 cells.

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  IHC staining of human ovary cancer using 84012-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84012-5-RR (SP100 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of human ovary cancer using 84012-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84012-5-RR (SP100 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with .

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  IF Staining of A431 using 84012-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 84012-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84012-5-RR bindet in WB, IHC, IF/ICC, ELISA SP100 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SP100 fusion protein Ag35193
• Vollständiger Name: SP100 nuclear antigen
• Berechnetes Molekulargewicht: 879 aa, 100 kDa
• Beobachtetes Molekulargewicht: 70-100 kDa, 110-140 kDa
• GenBank-Zugangsnummer: BC011562
• Gene symbol: SP100
• Gene ID (NCBI): 6672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.