SP110 Polyklonaler Antikörper

SP110 Polyklonal Antikörper für WB, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11502-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 11502-1-AP (same clone as 11502-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11502-1-AP (same clone as 11502-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11502-1-AP (same clone as 11502-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 11502-1-AP (same clone as 11502-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 11502-1-AP (same clone as 11502-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 11502-1-AP (same clone as 11502-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 11502-1-AP (same clone as 11502-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11502-1-AP (same clone as 11502-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 11502-1-AP (same clone as 11502-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 11502-1-AP (SP110 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 11502-1-AP (same clone as 11502-1-PBS)

  IP result of anti-SP110 (IP:11502-1-AP, 4ug; Detection:11502-1-AP 1:800) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 11502-1-AP (same clone as 11502-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SP110 (11502-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11502-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11502-1-PBS bindet in WB, FC (Intra), IP, Indirect ELISA SP110 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SP110 fusion protein Ag2042
• Vollständiger Name: SP110 nuclear body protein
• Berechnetes Molekulargewicht: 689 aa, 79 kDa
• Beobachtetes Molekulargewicht: 62-66 kDa
• GenBank-Zugangsnummer: BC019059
• Gene symbol: SP110
• Gene ID (NCBI): 3431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.