SP6 Polyklonaler Antikörper

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• KD/KO Validated

SP6 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21234-1-PBS

Synonyme

AI1K, EPFN, EPIPROFIN, KLF14, Krueppel-like factor 14

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  WB analysis of fetal human brain using 21234-1-AP (same clone as 21234-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21234-1-AP (same clone as 21234-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 21234-1-AP (same clone as 21234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 21234-1-AP (same clone as 21234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 21234-1-AP (same clone as 21234-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21234-1-PBS bindet in WB, IHC, IF/ICC, ELISA SP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SP6 fusion protein Ag15707
• Vollständiger Name: Sp6 transcription factor
• Berechnetes Molekulargewicht: 376 aa, 40 kDa
• GenBank-Zugangsnummer: BC103951
• Gene symbol: SP6
• Gene ID (NCBI): 80320
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.