SPA17 Polyklonaler Antikörper

SPA17 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13367-1-AP

Synonyme

Cancer/testis antigen 22, CT22, SP17, SP17 1, SPA17, sperm autoantigenic protein 17, Sperm protein 17, Sperm surface protein Sp17

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 13367-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13367-1-AP (SPA17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13367-1-AP

  IP Result of anti-SPA17 (IP:13367-1-AP, 3ug; Detection:13367-1-AP 1:400) with mouse testis tissue lysate 4800ug.

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  IHC staining of human testis using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 13367-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13367-1-AP bindet in WB, IP, IHC, IF, ELISA SPA17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPA17 fusion protein Ag4186
• Vollständiger Name: sperm autoantigenic protein 17
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC032457
• Gene symbol: SPA17
• Gene ID (NCBI): 53340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.

Publikationen

Species	Application	Title
human	IHC	Br J Cancer; Tumour antigen expression in hepatocellular carcinoma in a low-endemic western area.; Authors - K Sideras
human	WB,IF	Front Immunol; Sperm Autoantigenic Protein 17 Predicts the Prognosis and the Immunotherapy Response of Cancers: A Pan-Cancer Analysis.; Authors - Zewei Tu
mouse	WB	Sci Rep; Testis-specific peroxiredoxin 4 variant is not absolutely required for spermatogenesis and fertility in mice.; Authors - Takujiro Homma
human	IHC	BMC Cancer; Validity and prognostic significance of sperm protein 17 as a tumor biomarker for epithelial ovarian cancer: a retrospective study.; Authors - Laurie L Brunette
human	WB	Asian J Androl; Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation; Authors - Kun Zhang