SPA17 Polyklonaler Antikörper

SPA17 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13367-1-PBS

Synonyme

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  WB analysis of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13367-1-AP (SPA17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13367-1-AP (same clone as 13367-1-PBS)

  IP result of anti-SPA17 (IP:13367-1-AP, 3ug; Detection:13367-1-AP 1:400) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13367-1-AP (same clone as 13367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13367-1-AP (SPA17 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13367-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13367-1-PBS bindet in WB, IHC, IP, Indirect ELISA SPA17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPA17 fusion protein Ag4186
• Vollständiger Name: sperm autoantigenic protein 17
• Berechnetes Molekulargewicht: 151 aa, 17 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC032457
• Gene symbol: SPA17
• Gene ID (NCBI): 53340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sperm surface protein Sp17(SPA17)is a 151 amino acids protein (17kDa), which is a sperm surface peripheral membrane protein. It is a highly conserved mammalian protein expressed in developing spermatozoa and mature spermatids, and reported to play a role in the interaction of sperm with the zona pellucida. SP17 exists as a homodimer and localizes to the head and tail of spermatozoa. SP17 has been identified as a cancer/testis antigen and is expressed in ovarian cancer and multiple myeloma. This suggests that SP17 could be suitable as a target in tumor immunotherapy.