SPAG11A/B Polyklonaler Antikörper

SPAG11A/B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16770-1-PBS

Synonyme

SPAG11A, Antimicrobial-like protein Bin-1b, BIN1B, EP2, He2

---

• 

  WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 16770-1-AP (same clone as 16770-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A/B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16770-1-PBS bindet in WB, IHC, Indirect ELISA SPAG11A/B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG11A/B fusion protein Ag9846
• Vollständiger Name: sperm associated antigen 11A
• Berechnetes Molekulargewicht: 123 aa, 14 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC058833
• Gene symbol: SPAG11A
• Gene ID (NCBI): 653423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671). SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing. This antibody recognizes SPAG11A and SPAG11B, but not SPAG11, because SPAG11 is a different transcript.