SPAG4-Specific Polyklonaler Antikörper

SPAG4-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19721-1-PBS

Synonyme

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  WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 19721-1-AP (same clone as 19721-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19721-1-AP (SPAG4-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 19721-1-AP (same clone as 19721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SPAG4-Specific antibody (19721-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19721-1-AP (same clone as 19721-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 19721-1-AP (SPAG4-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19721-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19721-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA SPAG4-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: sperm associated antigen 4
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_003116
• Gene symbol: SPAG4
• Gene ID (NCBI): 6676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG4 may assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail. This antibody is specific to SPAG4. We observed that the molecular weight of SPAG4-Specific was 55 kDa.