SPAG4L Polyklonaler Antikörper

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• KD/KO Validated

SPAG4L Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 17495-1-AP

Synonyme

SPAG4L, SUN5, TSARG4

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Galerie der Validierungsdaten

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  WB analysis using 17495-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17495-1-AP (SPAG4L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 17495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 17495-1-AP (SPAG4L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 11 publications below

Produktinformation

17495-1-AP bindet in WB, IF, ELISA SPAG4L und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG4L fusion protein Ag11629
• Vollständiger Name: sperm associated antigen 4-like
• Berechnetes Molekulargewicht: 379 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC026118
• Gene symbol: SPAG4L
• Gene ID (NCBI): 140732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	WB	Am J Hum Genet; Biallelic SUN5 Mutations Cause Autosomal-Recessive Acephalic Spermatozoa Syndrome.; Authors - Fuxi Zhu
human	IF	Am J Hum Genet; Mutations in PMFBP1 Cause Acephalic Spermatozoa Syndrome.; Authors - Fuxi Zhu
mouse	WB,IF	Elife; Essential role for SUN5 in anchoring sperm head to the tail.; Authors - Yongliang Shang ; KO Validated
human	IF	Int J Mol Sci; Identification and Characterization of the Most Common Genetic Variant Responsible for Acephalic Spermatozoa Syndrome in Men Originating from North Africa.; Authors - Caroline Cazin
human	WB,IF	Cancers (Basel); Nuclear Membrane Protein SUN5 Is Highly Expressed and Promotes Proliferation and Migration in Colorectal Cancer by Regulating the ERK Pathway; Authors - Xiaoyue Song