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  • Featured Product
  • KD/KO Validated

SPAG4L Polyklonaler Antikörper

SPAG4L Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-17495

Synonyme

dJ726C3.1, SPAG4L, SUN5, TSARG4

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated SPAG4L Polyclonal an (CL488-17495)

    FC experiment of PC-3 using CL488-17495

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SPAG4L (CL488-17495) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCPC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-17495 bindet in FC (Intra) SPAG4L und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SPAG4L fusion protein Ag11629
Vollständiger Name sperm associated antigen 4-like
Berechnetes Molekulargewicht 379 aa, 43 kDa
Beobachtetes Molekulargewicht 43 kDa
GenBank-ZugangsnummerBC026118
Gene symbol SPAG4L
Gene ID (NCBI) 140732
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 SPAG4L antibody CL488-17495Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen