SPAG5 Polyklonaler Antikörper

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• KD/KO Validated

SPAG5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14726-1-PBS

Synonyme

sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest

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  WB analysis using 14726-1-AP (same clone as 14726-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  WB analysis using 14726-1-AP (same clone as 14726-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 14726-1-AP (SPAG5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

  IP result of anti-SPAG5 (IP:14726-1-AP, 5ug; Detection:14726-1-AP 1:1000) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 14726-1-AP (same clone as 14726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 14726-1-AP (SPAG5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14726-1-AP (same clone as 14726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14726-1-AP (SPAG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14726-1-AP (same clone as 14726-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPAG5 antibody (14726-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14726-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14726-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SPAG5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG5 fusion protein Ag6485
• Vollständiger Name: sperm associated antigen 5
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 135 kDa, 150 kDa
• GenBank-Zugangsnummer: BC000322
• Gene symbol: SPAG5
• Gene ID (NCBI): 10615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.