SPAG5 Rekombinanter Antikörper

SPAG5 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242374H6

Kat-Nr. : 84809-4-RR

Synonyme

Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest, Astrin

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  WB analysis using 84809-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84809-4-RR (SPAG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84809-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84809-4-RR against Human SPAG5 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, K-562-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84809-4-RR bindet in WB, ELISA SPAG5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: SPAG5 fusion protein Ag6485
• Vollständiger Name: sperm associated antigen 5
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: 135 kDa, 150 kDa
• GenBank-Zugangsnummer: BC000322
• Gene symbol: SPAG5
• Gene ID (NCBI): 10615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.