SPAG8 Polyklonaler Antikörper

SPAG8 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13915-1-PBS

Synonyme

HSD-1, HSD 1, CT142, CILD28, BS84

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  WB analysis using 13915-1-AP (same clone as 13915-1-PBS)

  DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 13915-1-AP (SPAG8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13915-1-AP (same clone as 13915-1-PBS)

  IP result of anti-SPAG8 (IP:13915-1-AP, 3ug; Detection:13915-1-AP 1:500) with mouse testis tissue lysate 6000ug. This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13915-1-AP (same clone as 13915-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13915-1-AP (SPAG8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 13915-1-AP (same clone as 13915-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SPAG8 antibody (13915-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13915-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13915-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA SPAG8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAG8 fusion protein Ag4910
• Vollständiger Name: sperm associated antigen 8
• Berechnetes Molekulargewicht: 45 kDa, 54 kDa
• Beobachtetes Molekulargewicht: 56-70 kDa
• GenBank-Zugangsnummer: BC019247
• Gene symbol: SPAG8
• Gene ID (NCBI): 26206
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAG8, also named as Sperm-associated antigen 8 or HSD-1, is a 426 amino acid protein, which is expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. SPAG8 may play a role in fertility and microtubule formation through interaction with RANBP9. SPAG8 as testis-specific protein is produced during male germ cell differentiation and functions in a germ cell type- and stage-specific manner during spermatogenesis.