SPAM1 Polyklonaler Antikörper

SPAM1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 11208-1-AP

Synonyme

HYA1, Hyal PH20, HYAL1, HYAL3, HYAL5, Hyaluronoglucosaminidase PH 20, PH 20, PH20, SPAG15, SPAM1, Sperm adhesion molecule 1, Sperm surface protein PH 20

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• 

  WB analysis of PC-3 using 11208-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11208-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11208-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, humanes Hodengewebe, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11208-1-AP bindet in WB, ELISA SPAM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAM1 fusion protein Ag1682
• Vollständiger Name: SPAM1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa
• GenBank-Zugangsnummer: BC026163
• Gene symbol: SPAM1
• Gene ID (NCBI): 6677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.

Publikationen

Species	Application	Title
bovine	WB	Int J Biol Macromol; TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.; Authors - Na Wang