SPARC Polyklonaler Antikörper

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• KD/KO Validated

SPARC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Kat-Nr. : 15274-1-AP

Synonyme

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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Galerie der Validierungsdaten

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  WB analysis of ROS1728 using 15274-1-AP

  A375 cells, ROS1728 cells, and human placenta tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15274-1-AP

  WB result of SPARC antibody (15274-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPARC transfected A375 cells.

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  WB analysis of human testis using 15274-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 15274-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ROS1728-Zellen, A375-Zellen, humanes Hodengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

15274-1-AP bindet in WB, IHC, IF, CoIP, ELISA SPARC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Sikahirsch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPARC fusion protein Ag7390
• Vollständiger Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35-43 kDa
• GenBank-Zugangsnummer: BC004974
• Gene symbol: SPARC
• Gene ID (NCBI): 6678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secreted Protein Acidic And Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. 

What is the molecular weight of SPARC?

The calculated molecular mass of SPARC is 35 kDa. SPARC is a glycoprotein consisting of 303 amino acids in the cytoplasm and 286 amino acids after the signal sequence is cleaved in secretion. 

Where is SPARC expressed?

Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis (PMID: 2440898). SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts.

Though previously described as being exclusively expressed in mineralized tissue, it is now understood that SPARC is more widely expressed and is associated with high levels of collagen deposition, found in the extracellular matrix (ECM) of a number of tissues.

What is the function of SPARC?

SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions (PMID: 26851678).

SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins (PMID: 19798598). It is known to regulate interactions between cells and the ECM, meaning it plays a role in many important processes such as cell migration and proliferation.

What diseases are associated with SPARC?

Overexpression of SPARC has been identified in a number of different types of cancers (PMID: 18849185), although its role may vary. For example, in neuroblastomas SPARC acts as a suppressor but in gliomas, it causes greater invasion of the tumor. Mutations in SPARC can also lead to Osteogenesis Imperfecta, also known as brittle bone disease (PMID: 26027498).

Publikationen

Species	Application	Title
human	WB	ACS Appl Mater Interfaces; Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.; Authors - Maichi Hama
human	IHC	Cancer Discov; PMID: 22585996; Authors - Frese Kristopher K KK
human	WB,IF	Theranostics; A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.; Authors - Lindsay B Alcaraz
rat	IHC	Clin Cancer Res; The pancreas cancer microenvironment.; Authors - Feig Christine C
human	IF	Int J Cancer; Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression; Authors - Zhenyu Liao
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani

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