SPARC Monoklonaler Antikörper

SPARC Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2C1

Kat-Nr. : 66426-1-PBS

Synonyme

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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  WB analysis of A375 using 66426-1-Ig (same clone as 66426-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66426-1-Ig (same clone as 66426-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66426-1-Ig (same clone as 66426-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66426-1-Ig (SPARC Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66426-1-Ig (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 66426-1-Ig (same clone as 66426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using SPARC antibody (66426-1-Ig, Clone: 1A2C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66426-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66426-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA SPARC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SPARC fusion protein Ag7390
• Vollständiger Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 52 kDa
• GenBank-Zugangsnummer: BC004974
• Gene symbol: SPARC
• Gene ID (NCBI): 6678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).