SPARC Monoklonaler Antikörper

SPARC Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2C1

Kat-Nr. : CL488-66426

Synonyme

Basement membrane protein 40, BM 40, ON, Osteonectin, SPARC

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  IF Staining of C6 using CL488-66426

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite® Plus 488 SPARC antibody (CL488-66426, Clone: 1A2C1 ) at dilution of 1:200.

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  FC experiment of A375 using CL488-66426

  1x10^6 A375 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SPARC Monoclonal antibody (CL488-66426, Clone:1A2C1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: C6-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66426 bindet in IF/ICC SPARC und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SPARC fusion protein Ag7390
• Vollständiger Name: secreted protein, acidic, cysteine-rich (osteonectin)
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 38 kDa, 52 kDa
• GenBank-Zugangsnummer: BC004974
• Gene symbol: SPARC
• Gene ID (NCBI): 6678
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).