SPARCL1 Polyklonaler Antikörper

SPARCL1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13517-1-AP

Synonyme

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

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Galerie der Validierungsdaten

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  WB analysis of human heart using 13517-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 13517-1-AP

  IP Result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug.

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  IHC staining of human tonsillitis using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 13517-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13517-1-AP bindet in WB, IP, IHC, IF, ELISA SPARCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPARCL1 fusion protein Ag4448
• Vollständiger Name: SPARC-like 1 (hevin)
• Berechnetes Molekulargewicht: 664 aa, 75 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC033721
• Gene symbol: SPARCL1
• Gene ID (NCBI): 8404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

Publikationen

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.; Authors - Hao Wang
		J Neurochem; Defective Synthesis and Release of Astrocytic Thrombospondin-1 Mediates the Neuronal TDP-43 Proteinopathy Resulting in Defects in Neuronal Integrity Associated with Chronic Traumatic Encephalopathy: In Vitro Studies.; Authors - A R Jayakumar
human	IHC	Cancer Biomark; Integrated analysis reveals down-regulation of SPARCL1 is correlated with cervical cancer development and progression; Authors - Dong-Ming Wu
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma
mouse	IF	PLoS One; Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility.; Authors - Ling Yu
mouse	WB	J Chem Neuroanat; Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.; Authors - Lite Ge