SPAST Polyklonaler Antikörper

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• KD/KO Validated

SPAST Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 22792-1-AP

Synonyme

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

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Galerie der Validierungsdaten

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  WB analysis using 22792-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22792-1-AP (SPAST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 22792-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPAST antibody (22792-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

22792-1-AP bindet in WB, IF, ELISA SPAST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPAST fusion protein Ag18797
• Vollständiger Name: spastin
• Berechnetes Molekulargewicht: 616 aa, 67 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC150260
• Gene symbol: SPAST
• Gene ID (NCBI): 6683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the growth and regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.

Publikationen

Species	Application	Title
human	WB	Cell Rep; Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.; Authors - Inna Goliand ; KO Validated
rat	WB	FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
human,mouse	WB,IF	Acta Neuropathol Commun; The enhanced association between mutant CHMP2B and spastin is a novel pathological link between frontotemporal dementia and hereditary spastic paraplegias; Authors - Yongping Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mhd Aghyad (Verified Customer) (09-15-2022)

I used the antibody for western blotting at 1:1000 dilution and it worked well. Clean clear bands could be observed at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK cells