SPAST Monoklonaler Antikörper

SPAST Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5A7

Kat-Nr. : 67361-1-PBS

Synonyme

ADPSP, FSP2, KIAA1083, SPAST, Spastic paraplegia 4 protein, spastin, SPG4

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  WB analysis using 67361-1-Ig (same clone as 67361-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67361-1-Ig (SPAST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67361-1-Ig (SPAST antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67361-1-Ig (same clone as 67361-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SPAST antibody (67361-1-Ig, Clone: 1F5A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67361-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67361-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA SPAST und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SPAST fusion protein Ag18866
• Vollständiger Name: spastin
• Berechnetes Molekulargewicht: 616 aa, 67 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC150260
• Gene symbol: SPAST
• Gene ID (NCBI): 6683
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPAST encodes a microtubule-severing protein called spastin. By severing microtubules into small segments, SPAST participates in the regeneration of neurites. Mutations in the SPAST gene (previously known as SPG4) are the most common causes of hereditary spastic paraplegia (HSP-SPG4). Mammalian cells express at least two spastin isoforms, a less-abundant full-length form (M1-spastin, 68 kDa) and a more highly expressed isoform (M87-spastin, 60 kDa) that lacks the N-terminal 86 amino acids of the full-length protein. Also, there are two additional 64 kDa and 55 kDa isoforms due to alternative splicing of SPG4 exon 4. This antibody recognizes all these isoforms of spastin.