SPATA22 Polyklonaler Antikörper

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• KD/KO Validated

SPATA22 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16989-1-PBS

Synonyme

NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20

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  WB analysis of mouse testis using 16989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 16989-1-AP (same clone as 16989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16989-1-PBS bindet in WB, IHC, IP, ELISA SPATA22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATA22 fusion protein Ag10591
• Vollständiger Name: spermatogenesis associated 22
• Berechnetes Molekulargewicht: 363 aa, 41 kDa
• Beobachtetes Molekulargewicht: 44, 41 kDa
• GenBank-Zugangsnummer: BC029483
• Gene symbol: SPATA22
• Gene ID (NCBI): 84690
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.