SPATA7 Polyklonaler Antikörper

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• KD/KO Validated

SPATA7 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12020-1-AP

Synonyme

HSD 3.1, HSD3, SPATA7, spermatogenesis associated 7

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  WB analysis using 12020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12020-1-AP (SPATA7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12020-1-AP

  IP result of anti-SPATA7 (IP:12020-1-AP, 3ug; Detection:12020-1-AP 1:300) with mouse testis tissue lysate 8000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12020-1-AP bindet in WB, IP, IF, IHC, ELISA SPATA7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATA7 fusion protein Ag2649
• Vollständiger Name: spermatogenesis associated 7
• Berechnetes Molekulargewicht: 599 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC008656
• Gene symbol: SPATA7
• Gene ID (NCBI): 55812
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPATA7, also named as HSD3, may be involved in retinal function. It is interesting to note that mutations in SPATA7 cause LCA and RP/ARRP , two overlapping but distinct human retinal diseases. SPATA7 is another LCA and Juvenile RP Gene.(PMID:19268277) It is a highly conserved, vertebrate-specific protein which expressed in the mature retina. For some modification, the MW always migrate 5-8kd. The antibody can reoginze all the 3 isoforms of SPATA7.

Publikationen

Species	Application	Title
mouse	IF, IHC	Am J Hum Genet; Mutations in SPATA7 cause Leber congenital amaurosis and juvenile retinitis pigmentosa.; Authors - Wang Hui H
mouse	WB	Hum Mol Genet; Spata7 is a retinal ciliopathy gene critical for correct RPGRIP1 localization and protein trafficking in the retina.; Authors - Aiden Eblimit ; KO Validated
mouse	WB,IF	Gene Ther; AAV8(Y733F)-mediated gene therapy in a Spata7 knockout mouse model of Leber congenital amaurosis and retinitis pigmentosa.; Authors - H Zhong ; KO Validated
mouse	WB	Exp Eye Res; Conditional loss of Spata7 in photoreceptors causes progressive retinal degeneration in mice.; Authors - Aiden Eblimit