SPATS2 Polyklonaler Antikörper

SPATS2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32517-1-PBS

Synonyme

Nbla00526, p59scr, SCR59, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10

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  WB analysis using 32517-1-AP (same clone as 32517-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 32517-1-AP (SPATS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 32517-1-AP (same clone as 32517-1-PBS)

  IP result of anti-SPATS2 (IP:32517-1-AP, 4ug; Detection:32517-1-AP 1:5000) with HeLa cells lysate 1400 ug. This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 32517-1-AP (same clone as 32517-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SPATS2 antibody (32517-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 32517-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

32517-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA SPATS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPATS2 fusion protein Ag38507
• Vollständiger Name: spermatogenesis associated, serine-rich 2
• Berechnetes Molekulargewicht: 60kDa，545aa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: NM_001293285.1
• Gene symbol: SPATS2
• Gene ID (NCBI): 65244
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPATS2, also known as SCR59 and SPATA10, is a member of the RNA-binding proteins whose aberrant expression is associated with carcinogenesis in several types of cancer. It is mainly expressed in the adult testis, with low expression in liver and other tissues (PMID: 33037180). The apparent molecular weight is higher than the calculated molecular weight of 60 kDa, probably due to post-translational modification, probably by phosphorylation.